Journal: Data in Brief

Article Title: Dataset on the activation of Müller cells through macrophages upon hypoxia in the retina

doi: 10.1016/j.dib.2017.11.062

Figure Lengend Snippet: Antibodies used.

Article Snippet: Rabbit anti-CD11b , 1:100 , Novus Biologicals , NB110-89474.

Techniques: Concentration Assay

Journal: Data in Brief

Article Title: Dataset on the activation of Müller cells through macrophages upon hypoxia in the retina

doi: 10.1016/j.dib.2017.11.062

Figure Lengend Snippet: Validation of the VEGF fl/fl LysMCre mouse model in the retina. (A) Relative Cre mRNA expression levels in the retina. Measurement of relative Cre mRNA expression levels by quantitative Real-Time PCR in VEGF mcko and control mice under normoxic and hypoxic conditions (animal numbers per group are indicated in the figure on each bar) as an indirect indication of the occurrence of recombination in the retina on P14 (VEGF mcko hypoxia 5.48×10 −4 ±5.56×10 −4 ; VEGF mcko normoxia 6.63×10 −4 ±10.06×10 −4 ) and on P17 (VEGF mcko hypoxia 6.46×10 −4 ±6.85×10 −4 ; VEGF mcko normoxia 11.13×10 −4 ±18.58×10 −4 ). (Cre mRNA expression in the control tissues brain and bone marrow C, D). (B) Genomic recombination efficiency in various murine tissues. Detection of the length of the VEGF-A gene in thyme (A), brain (B), spleen (C) and bone marrow (D) in three mice per group of VEGF mcko (VEGF fl/fl Cre tg/+ ) and two types of control animals through genomic PCR analysis. DNA from the bone marrow and spleen was isolated after magnetic cell sorting of CD11b positive cells. A 420 bp fragment in VEGF mcko mice (VEGF fl/fl Cre tg/+ ) indicates the occurrence of recombination, a 1934 bp long fragment was found in the floxed and a 1866 bp long fragment in the wildtype control animals. (C) Screen for Pdeb rd1 mutation. Genomic PCR screen for a Pdeb rd1 mutation causing postnatal photoreceptor degeneration in three VEGF mcko (VEGF fl/fl Cre tg/+ ) and three control animals (VEGF fl/fl Cre +/+ ). A 400 bp long fragment was proof of the presence of a wildtype allele and ruled out the presence of the mutation, which would have been indicated by a 550 bp long band.

Article Snippet: Rabbit anti-CD11b , 1:100 , Novus Biologicals , NB110-89474.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Isolation, FACS, Mutagenesis

Journal: Data in Brief

Article Title: Dataset on the activation of Müller cells through macrophages upon hypoxia in the retina

doi: 10.1016/j.dib.2017.11.062

Figure Lengend Snippet: Histochemistry of CD11b positive cells in spleen sections. Representative staining of CD11b positive cells in spleens of control mice kept under hypoxic conditions. Clodronate injection (right) led to a depletion of CD11b positive cells whereas sections of PBS injected controls (left) still showed a considerable amount of CD11b positive cells (dark staining).

Article Snippet: Rabbit anti-CD11b , 1:100 , Novus Biologicals , NB110-89474.

Techniques: Staining, Injection

Journal: Data in Brief

Article Title: Dataset on the activation of Müller cells through macrophages upon hypoxia in the retina

doi: 10.1016/j.dib.2017.11.062

Figure Lengend Snippet: Presence of mononuclear phagocytes (MPs) in close proximity to blood vessels. Representative images for mononuclear phagocytes (CD11b in red) and vessels (AF488-Isolectin-IB4 in green) of retinal flatmount preparations on P14 and P17 under hypoxic conditions in control mice.

Article Snippet: Rabbit anti-CD11b , 1:100 , Novus Biologicals , NB110-89474.

Techniques:

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Aryl Hydrocarbon Receptor Activation in Astrocytes by Laquinimod Ameliorates Autoimmune Inflammation in the CNS

doi: 10.1212/NXI.0000000000000946

Figure Lengend Snippet: (A) EAE was induced in control and GFAP-AHR (GFAP Cre AHR fl/fl ). Animals were treated with daily oral doses of laquinimod or vehicle starting from day 2 after immunization. Clinical scores are mean ± SEM and representative of 2 independent experiments. * p < 0.05. ** p < 0.01. *** p < 0.001 by 2-way analysis of variance (ANOVA). (B) Absolute numbers of CNS-infiltrating proinflammatory monocytes as determined by fluorescence-activated cell sorting (FACS) staining for CD11b, CD45, and Ly6C. Data are mean ± SEM. (C) Absolute numbers and (D) relative fractions of CNS-infiltrating T cells were determined by FACS staining for CD3, CD4, IFN-γ, and IL-17A. Data are mean ± SEM of n = 5 mice per group and representative of 3 independent experiments. AHR = aryl hydrocarbon receptor; EAE = experimental autoimmune encephalomyelitis; GFAP = glial fibrillary acidic protein; IFN = interferon; IL = interleukin; n.s. = not significant as determined by 1-way ANOVA followed by the Tukey post hoc test.

Article Snippet: Mononuclear cells were isolated from the CNS as previously described, and astrocytes, monocytes, and microglia were sorted as described before., Isolated CNS cells were stained with fluorochrome-conjugated antibody to CD11b (M1/70, 1:100), CD45 (90, 1:100), Ly6C1 (HK1.4, 1:100), CD105 (N418, 1:100), CD140a (APA5, 1:100), CD11c (N418, 1:100), F4/80 (BM8, 1:50), O4 (O4, Miltenyi Biotec, 1:10), and CD19 (eBio1D3, 1:100).

Techniques: Control, Fluorescence, FACS, Staining